Part:BBa_K2267008

Cyclic dimeric guanosine monophosphate (c-di-GMP)

Cyclic dimeric guanosine monophosphate (c-di-GMP) is a new type of second messenger molecule that is ubiquitous in bacteria. Bacteria utilize it to perceive cell surface signals and activate targets within cells, which involves amplification of the original signal and the subsequent expression of a series of specific genes in the cell. These genes affects the various physiological and biochemical processes in the cells. c-di-GMP control a wide range of process, and has a role in transcription, translation and post-translation levels. In addition, c-di-GMP participates in the regulation of multiple biological functions and affects biofilm formation. In a nutshell, it is a crucial elements to the formation of membrane-like bacterial cellulose and the improvement of BC production.

Expressed

Figure 2. Agarose Gel Electrophoresis for c-di-GMP DNA

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 964
  Illegal SpeI site found at 2
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 964
  Illegal SpeI site found at 2
  Illegal NotI site found at 9
  Illegal NotI site found at 970
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 964
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 964
  Illegal SpeI site found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 964
  Illegal SpeI site found at 2
  Illegal NgoMIV site found at 404
  Illegal AgeI site found at 167
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 494